CHEM543 (3-0-3)

SEPARATION METHODS

Syllabus

 

Instructor:      Khalid Alhooshani

Office: 4-223 ; Phone: 860-3065

Email: hooshani@kfupm.edu.sa

Lectures:        Sunday and Tuesday from 6:30 PM to 7:45 PM

References:    The Essence of Chromatography by Colin Poole (Elsevier)

Themes:          1. Fundamental concepts of chromatography

2. Gas Chromatography

3. Liquid Chromatography

4. Other Analytical Separations

Homework: Problems will be assigned throughout the semester as an aid in comprehending the course material.  Two homework will be given.

Quiz and Exams: Two quizzes will be given throughout the semester as an aid to review the course material periodically.  Two exams will be included in the course.  The midterm exam covers the first and second part of the themes.  The final one is a comprehensive exam, but it will emphasize the last two parts of the themes. The Final exam will be on  Sunday   June 8, 2008 7:00 PM

Research Proposal and Presentation:  The topic of the proposal is on a separation technique.  The length of the proposal is about 2000 words.  The detailed requirement will be given after the midterm exam. The presentation is the oral version of the proposal: 15-20 min talk.

LECTURE SCHEDULE

(Sunday and Tuesday, 6:30 pm to 7:45 pm, Room 106, Beld 4)

Date Topic
Feb. 17, 19 Introduction, analytical separation techniques and their classification, chromatographic and electrophoretic separation techniques, importance of separation techniques in science and industry, historical background
Feb. 24, 26 Chromatographic separation methods, mobile and stationary phases in chromatography, chromatographic separation mechanism at the molecular level, definitions and fundamental concepts of chromatography

March 2, 4 Gas chromatography (GC), column technology, separation mechanism, band broadening in GC, column efficiency, van Deemter equation and plot, Golay equation, separation factor, resolution

March 9, 11 Sample introduction in GC,Split. injection, split/splitless injection, on-column injection, sample capacity of packed and open tubular columns in GC, sample introduction in quantitative analysis and trace analysis

March 16 Quiz #1
March 23, 18 GC detectors: Fundamental concepts, flame ionization detector, thermal conductivity detector, electron capture detector, nitrogen-phosphorus detector

March 25,  30 High Performance Liquid Chromatography (HPLC): normal phase and reversed-phase HPLC, Retention mechanisms in HPLC, instrumentation for HPLC, selection of mobile phase in HPLC

April  1st , 6 Stationary phases in HPLC, surface deactivation of silica based packing material; monomeric, polymeric, and polymer-encapsulated packing materials, Sample introduction and detection technique in HPLC.
April   8 Midterm Exam
April  12- 16 Midterm Vacation

April   20, 22

Mass spectrometric Detectors for chromatographic techniques
April  27, 29 Supercritical Fluid Chromatography (SFC): supercritical fluids as mobile phases in chromatography, advantages and drawbacks, Instrumental aspects of SFC
May   4, 6 Capillary Electrophoresis (CE): Theoretical basis of electrophoretic Separations, advantages, electrical double layer at the silica/aqueous buffer interface, electro osmotic flow (EOF).

May  11 CZE instrumentation, sample introduction and detection in CZE, column technology, band broadening in CZE, buffer selection in CZE. Electrokinetic chromatography: EOF as a means of mobile phase pumping in chromatography, Capillary Electrochromatography (CEC), advantages of CEC over HPLC and MEKC

May   13

Sample Preparation & Extraction Technology
May   18 Quiz #2
May  25, 27 Student Presentation
Jun    2, 4 Student Presentation
Jun 15 Final Exam